Hot!Rotifer Culture

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Fishtal
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Rotifer Culture

Culturing Live Rotifers
 

Live rotifers are the most common "first food" for larval fishes.  Unfortunately, rotifers are only nutritious if they are fed nutritious food.  First we will deal with culturing protocols and then move on to enrichment.
 

 
Things you'll need:
 
  • 5 Gallon buckets
  • Flexible airline tubing
  • Rigid airline tubing
  • Air pump
  • Rotifer starter culture
  • Food (Instant Algae, live Phytoplankton, etc.)
  • 60CC Syringe (Available at your local pharmacy.)
  • 120 micron strainer
  • 53 micron strainer
 

 
First, a little info on rotifers before I get into the specifics of technique.  
  • Rotifers hatch in 12hrs and are sexually mature at 18hrs
  • Female lifespan at 25degC is 6-8days (males only 2days.)
  • Largely classified as into two strains L & S strain (B. plicatillis & rotundiformis respectively.)
  • Brachionus Plicatillis is the most common
  • Best growth occurs at salinity between 1.007-1.014
  • Abrupt salinity change of 1.007 or more will cause mortality
  • Optimum temp is 18-25degC  (Room temp in the fish room works well for me.)
  • pH should be between 6.5-8.0
  • Free Ammonia below 1mg/l
  • For larval feeding suggest feeding nannochloropsis with 20% tetraselmis for optimal HUFA content.  "Rotifer Diet HD" from Reed Mariculture is what I use.
  • Rotifers do not need light to grow

 
Culturing Method
 
There are many ways to raise rotifers, the following is how I do it. 
 
Step 1)  Obtain a starter culture from a local source if possible.  Starter cultures are available online as well:  [link=http://www.mariculturetechnology.com/Foods.htm%3C/a%3E%3C/font%3E]http://www.mariculturetec...htm%3C/a%3E%3C/font%3E[/link]
Step 2)  Place the starter culture in a 5 gallon bucket.  Add enough fresh saltwater with a specific gravity of 1.015 to double the water volume in the bucket.  Feed with IA using the recipe below.
Step 3)  Insert a rigid airline tube connected to an air pump to provide aeration.
 
 
Step 4)  On day 2, add enough saltwater to double the volume again. 
#1

Replies (12) Related Threads

    Fishtal
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    Re:Rotifer Culture (permalink)
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    Rotifers in action:
     

     
    [video]http://www.youtube.com/watch?v=FRKGBw-4YUw[/video] 
     
    [video]http://www.youtube.com/watch?v=V-MtXmNLdRM[/video]
    #2
    clownfishman
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    Re:Rotifer Culture (permalink)
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    Tal, for the IA RotiferDiet, are you refering to the Reef Nutrition line or the RotiferDiet 3600 line?  They are of different concentration.
    #3
    Fishtal
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    Re:Rotifer Culture (permalink)
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    Umm_fish?
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    Re:Rotifer Culture (permalink)
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    For my rots I use a more expensive food, Reed's RotiGrow Plus, but it's fantastic. I've had no culture crashes in the months since starting with it, and the rots don't need enrichments before they get used. My cultures average out to about 50/mL at 1 mL of rot food per gallon of culture given twice per day (at SG 1.025) without a whole lot of effort. I've just started increasing the food a little to see if I get more concentrated cultures.
    #5
    Fishtal
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    Re:Rotifer Culture (permalink)
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    I guess I should've mentioned that I also use Reed's T-ISO.  I switch between the two products.  One in the morning and the other at night.
    #6
    THEJRC
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    Re:Rotifer Culture (permalink)
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    you guys and your refrigerated algaes..... yuck... 

    Awesome work tal! you got it up at your site as well?    
    Because it's all about the plankton!
    ~J      
    #7
    Fishtal
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    Re:Rotifer Culture (permalink)
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    THEJRC

    you guys and your refrigerated algaes..... yuck... 

    Awesome work tal! you got it up at your site as well?    


    I use the IA for rotifers as it's easier for me.  I do use live phytoplankton for greenwater.
     
    Actually, I pulled this from my site.
    #8
    Umm_fish?
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    Re:Rotifer Culture (permalink)
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    you guys and your refrigerated algaes..... yuck...



    Ah, you crack me up, Joe. I need to get a new bag of my goop if I ever get over this throat thing. (Back to the doc today after a whole day lost to pain. Back on steroids again so feeling better again.) I'll give you some to play with. It's really nasty in a lovely high-HUFA sort of way. 
    #9
    Gomojoe
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    Re:Rotifer Culture (permalink)
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    So what difficulties do you guys run into using the live phyto vs. the refrigerated kind?  I would imagine that the refrigerated kinds are more concentrated and probably a good blend of algeas for good nutrition, but if you are already cultivating live phtyo, why buy the other stuff?
    #10
    Umm_fish?
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    Re:Rotifer Culture (permalink)
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    Live phyto is a lot more work and is subject to culture crashes. And because of that, honestly, is just about as expensive as algae paste. Also, you don't have a lot of quality control on a live phyto culture grown in your house. 

    Paste algae is expensive-ish (but see above). Quality control is good. You probably should use an ammonia blocker with it (something like ClorAmX). It's just flat-out easier to maintain rot cultures with paste.
    --Andy
    #11
    shano
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    Re:Rotifer Culture (permalink)
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    this is very good post
    #12
    tazy
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    Re:Rotifer Culture (permalink)
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     I would imagine that the refrigerated kinds are more concentrated and probably a good blend of algeas for good nutrition, but if you are already cultivating live phtyo, why buy the other stuff?
    Cut down your
    JN0-633 exam questions stress by using our latest NS0-504 dumps exam and high quality pmc.edu and testking nec demos.
    #13
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