Hot!Copepod Culturing

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cmpenney
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Copepod Culturing

This excerpt is from an article in Advanced Aquarist.com written by Frank Marini & Dwayne Sapp:
http://www.advancedaquarist.com/issues/feb2003/breeder2.htm 
  
 
Parts:

The first step, as in any project, is to assemble the parts we will need for the project.  
 
1 – 10 gal tank
1 – Small air pump
1 – 2 gang air valve  
1 – 36" ¼" rigid tube (cut in two equal pieces)
1 – 12" section of ¼" airline
1 – 24" section of ¼" airline
1 – 36" section of ¼" airline
1 – Acrylic lid with holes for rigid tube 

Assembly:
The next step is to assemble the parts.
1)      Connect the air pump to the gang valve with the 12" section of ¼" airline.
2)      Connect each gang valve to the two remaining sections of airline.
3)      Connect a piece of rigid tubing to the ends of the two airline sections. 
   
 
4)      Insert the rigid tubing in two of the holes in the acrylic lid. Place them at opposite ends of the tank. This will allow for better flow through the tank. 
  
 

Select a source for phytoplankton to feed the Copepods 
 
Phytoplankton can be found from many sources, including home culturing. Commercially available phytoplankton will tend to be more concentrated than home grown cultures. Pictured here are examples of DT's Live Marine Phytoplankton,  Reed Mariculture's Plankton Live FeedDiet, and the typical 2-liter bottle of home grown phytoplankton. Which ever source you decide to use, make sure you use according to instructions to prevent contamination and spoilage.
Nannochlroposis is my preferred phytoplankton to feed copepods. Others may work as well or better, but Nannochlropsis is widely available from both commercial and home grown sources.

Once we have the phytoplankton, we need to fill the culture tank with an appropriate amount of phytoplankton. To prevent spillage and some of the mess, I typically fill the tank less than half full. 
   
 
Now we have the culture tank filled and ready for copepods, not just yet. We need to make sure the culture tank parameters are within ranges.


Temperature – For culturing copepods, I do not use a tank heater. I have had great success with room temperature. So what is room temperature? It means a room that is typically considered comfortable. This does not include the room with no insulation on the Northwest side of a house on the plains of North Dakota in January.
Salinity – It is best to match the culture tank to the tank to be fed. This helps eliminate the possibility of salinity shock for the copepods.
Airflow – With the culture tank filled with phytoplankton, we can set up the airflow. This does not need to be forceful, but does have to provide some circulation. I found that adjusting the airflow to a rate slow enough to count the bubbles to be adequate.
Lighting – Ambient room lighting or low wattage fluorescent lighting. 
 
Adding the Copepods:
If we've successful
Chad Penney - MASM President
My Build Journal
Agis quod Adis
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Replies (4) Related Threads

    Fishtal
    The Prez
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    Re:Copepod Culturing (permalink)
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    Here is an excellent podcast about copepods on TalkingReef.com with Adelaide Rhodes:  http://www.talkingreef.com/forums/podcast-episodes/2140-copepods-podcast-episode-69-a.html
    #2
    clamdigr
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    Re:Copepod Culturing (permalink)
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    Podcast? No pun intended eh....
    -Mike-

    240g In-wall - 48x48x24 - Mixed Reef  
    #3
    Fishtal
    The Prez
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    Re:Copepod Culturing (permalink)
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    clamdigr

    Podcast? No pun intended eh....


    LOL, I missed that one.  No pun intended, but it's great to listen to.  I'm actually still listening.
    #4
    mbetter95
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    Re:Copepod Culturing (permalink)
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    I do also culture Tetrraselmis but it is Nanno in the pics.  BTW, the rotifer videos belong in the rotifer thread, my mistake, they'll be moved.
    #5
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